FLNB and bone disorder: Previous studies showed that autosomal-dominant mutations of FLNB leading to AO1/AO3/BD/LRS did not alter protein levels in HEK293 cells [8], but they ignored the possibility that FLNB might exert its function in a cell-dependent way, and as FLNB variants only led to skeletal diseases, it was more rational for researchers to perform in vitro studies in a bone-derived cell line rather than HEK293.