After analyzed by ROC, we found that the AUCs of anti-CAGE, anti-GAGE7, anti-GBU4-5, anti-MAGE A1, anti-P53, anti-PGP9.5, and anti-SOX2 for the diagnosis of LC were all higher than 0.5. The gene discussed is SOX2; the disease is laryngotracheoesophageal cleft.