To confirm the LC–MS-identified RNF8 interactome, we chose YBX1, a critical regulator of transcription and translation that is widely recognized as an oncogenic driver in several solid tumors such as breast cancer [24, 25], which was only identified by LC–MS and not in 5 PPI databases (Fig. 6A) as the proof-of-concept target. Here, RNF8 is linked to breast cancer.