To examine TOP2B binding in the gene regulatory regions of differentially expressed genes such as NOTCH1, we used an existing TOP2B ChIP-seq data set derived from glioma cell lines TS543 and BT142 [62] along with publicly available data for CTCF, open chromatin (ATAC-seq), active promoters (H3K4Me3), and promoter/enhancer regions (H3K27Ac), all derived from SH-SY5Y cells or clonally related lines (SK-N-SH or SHEP). This evidence concerns the gene NOTCH1 and central nervous system cancer.