Rats with CCl4-induced cirrhosis showed significantly higher renal expression of both Na–K-Cl co-transporter 2 (NKCC2) and sodium-chloride symporter (NCC) transporters compared to normal negative control rats (p < 0.001), Figs. 6A, 7A. Regarding the effect of different extracts on NKCC2, all extracts, except for At-rt, significantly decreased renal expression of NKCC2 with the most prominent effect observed with the highest tested dose of At-ar. Here, SLC12A3 is linked to Cirrhosis.