Transient knockdown of GLI1 and GLI2 reduced HHIP-AS1 and HHIP expression in all of the above-mentioned SHH-cancer cell models (Fig. 2b and S2), while on the other hand overexpression of GLI1 (Fig. S2d) resulted in increased expression of HHIP and HHIP-AS1 (Fig. S3b), confirming HHIP-AS1 as a target gene of SHH signaling. Here, SHH is linked to cancer.