We found that besides TNFRSF4, Treg functional signature genes such as FoxP3, CTLA4, TIGIT, TNFRSF18 (GIRT), CCR8, LAYN, and MAGEH1 were also overexpressed in C11-Tregs-FoxP3 of treatment-naive and non-MPR tumor lesions, but not for MPR tumor lesions (Fig. 5C). Here, LAYN is linked to neoplasm.