SPHK1 and non-small cell lung carcinoma: In other primary NSCLC cells (pNSCLC-2 and pNSCLC-3) and immortalized lines (A549 and NCI-H1944), the SphK1/2 dual blocker dose-dependently induced cell death in the primary and immortalized NSCLC cells, evidenced by the results from the Trypan blue-staining assays (Fig. 2I).