Furthermore, in addition to having low variant allele frequencies for both variants, the EGFR c.2369C > T p.(Thr790Met) is associated with acquired resistance to EGFR tyrosine kinase inhibitors (EGFR-TKIs) [26] and would be expected to be present at a lower level than the original sensitising mutations in a tumour. This evidence concerns the gene EGFR and neoplasm.