We first evaluated PVT1 expression at baseline using primers spanning PVT1 transcript isoforms containing–exon 1–2, exon 2–3, and exon 6–7 (Fig S1B) in a panel of human ovarian cancer (OVCA) cells lines, normal immortalized fallopian tube epithelial cells (p211) and surface epithelial cells (IOSE80) (Fig 2A). Here, PVT1 is linked to ovarian cancer.