This effect of GFI1 overexpression is not cell type specific because GFI1 overexpression caused similar morphological changes in other lung cancer cell lines, including H460 and H1299, breast cancer cell lines MCF7 and MDA-MB-231, ovarian cancer cell line SKOV3, and colorectal cancer cell line HT-29 (Figure 2B and Supplemental Figure 3A). Here, GFI1 is linked to breast cancer.