GFI1 and cancer: As a third test of anchorage sensing, we examined the effect of GFI1 on anchorage-independent proliferation and found that GFI1-expressing cancer cells (A549-GFI1, H460-GFI1, H1155) formed significantly more colonies in soft agar than GFI1-negative cancer cells (A549, H460, H1155-GFI1-KO) (Figure 2G and Supplemental Figure 9B).