Reverse transcription–PCR (RT-PCR) and immunoblot in a subset of selected cells confirmed the higher level of GFI1 expression in SCLC lines (H526, H69, and H82) and in neuroendocrine NSCLC line H1155, all of which proliferate as clusters in suspension in vitro, compared with primary HUVECs, an immortalized line of human bronchial epithelial cells (HBECs), and lung adenocarcinoma cell lines (A549 and H460), which proliferate as adherent single cells (Figure 1C). The gene discussed is GFI1; the disease is lung adenocarcinoma.