We overexpressed GFI1 in anoikis-susceptible cells, including HUVECs and HBECs, lung cancer cells (A549, H460, H1299), ovarian cancer cells (SKOV3, OVCA432), liver cancer cells (HepG2, Hep3B), breast cancer cells (MDA-MB-231, MCF7), and colorectal cancer cells (HT-29, HCT8), and subsequently forced the cells into suspension by culturing them on low-attachment plates. The gene discussed is GFI1; the disease is breast carcinoma.