Similarly, positive effect predictions for E and P sequence classes were validated by previously studied mutations: an α-thalassemia mutation near HBM (chromosome 16: 209,709 T>C)32 known to create a GATA1 binding site and increase intergenic transcription was predicted to increase erythroblast-specific E12 activity; a TERT mutation found in individuals with familial melanoma (chromosome 5: 1,295,161 T>G)33 was predicted to increase P activity. Here, TERT is linked to melanoma.