The top 15 KEGG pathways were as follows: human cytomegalovirus infection, Kaposi sarcoma-associated herpesvirus infection, AGE-RAGE signaling pathway in diabetic complications, Hepatitis B, chagas disease (American trypanosomiasis), relaxin signaling pathway, human immunodeficiency virus 1 infection, fluid shear stress and atherosclerosis, prostate cancer, endocrine resistance, TNF signaling pathway, colorectal cancer, IL-17 signaling pathway, HIF-1 signaling pathway, and Epstein-Bar virus infection. The gene discussed is TNF; the disease is Chagas disease.