The accuracy of S100B combined with IL-1β, IL-2, IL-4, and IL-10 in classifying GAD and HC was 91.06 ± 2.42% using SVM, 92.73 ± 3.82% using RF, and 94.47 ± 2.06% using BPNN-Bagging (Table 3). Here, S100B is linked to generalized anxiety disorder.