The levels of miR-21 were found to be upregulated and the levels of PTEN were downregulated in the pancreatic cancer cell lines, such as CAPAN-1, BxPC-3, JF305, PANC-1, and SW1990, when compared with those in normal human pancreatic HPDE6-C7 cells. Here, PTEN is linked to familial pancreatic carcinoma.