To validate some of the differentially expressed genes identified, we focused our attention on two different ccRCC cell lines (Caki1 and A498); we stimulated the cells with increasing amounts of IL1β for 4 h, and we measured the expression levels of NFKBIZ as the most distinctive transcription factor of the IL1R and TLR signaling pathways that emerged from RNA-sequencing. This evidence concerns the gene NFKBIZ and nonpapillary renal cell carcinoma.