Doublecortin-like kinase 1 (DCLK1), involved in the epithelial-mesenchymal transition (TME) and tumor progression, is a novel target for CRC immunotherapy and anti-DCLK1 CAR T-cells resulted in cytotoxicity and secretion of IFN-γ after incubation with CRC cells in two. The gene discussed is DCLK1; the disease is colorectal carcinoma.