Anti-EpCAM CAR T-cells used against CRC cells and models exhibited cytotoxic lysis of the targeted cells that secreted cytotoxic cytokines, including IFN-γ and tumor necrosis factor-alpha (TNF-α), resulting in tumor growth and development in xenograft mouse models (Zhang et al., 2019). The gene discussed is TNF; the disease is colorectal carcinoma.