Using the third generation CARs with CD3z-CD28-4-1BB as multiple domains, targeting the envelope glycoprotein GP120 (gp120) and anti-gp120 CAR T-cells in HIV infection showed increased effectiveness in lysing Env-expressing cells in vitro compared to CD4ζ CAR T-cells (Liu et al., 2016). Here, ERVW-1 is linked to HIV infectious disease.