In light of this, an initial study focusing (NCT01355695) on toxicity assessment was conducted using T-cells with transient expression of second-generation murine anti-mesothelin CAR containing CD3ζ and 41BB signaling domains (Maus et al., 2013; Beatty et al., 2014); in phase I safety trial none of the patients exhibited “on-target, off-tumor” toxicity post-infusion, and there was no evidence of clinical responses (Beatty et al., 2014; Klampatsa et al., 2017). The gene discussed is MSLN; the disease is neoplasm.