The addition of IL-1β (100 pg/ml) significantly increased ALP activity in MSCs compared to OM alone at both time-points analysed (1.13 ± 0.02 fold change compared to OM at day 7 and 1.14 ± 0.02 fold change compared to OM at day 10) (Figure 3A). The gene discussed is IL1B; the disease is ocular melanoma.