Post-mortem brains were examined for neuropathologic features of Alzheimer disease and related disorders.38 Exclusion criteria included Lewy body dementia, Parkinson disease, hippocampal sclerosis, vascular disease and large strokes.35,37,39,40 Apolipoprotein E (APOE) genotyping was performed as previously reported35,37,39,40 and confirmed by RNA-Seq to identify nonsynonymous polymorphisms encoding base substitutions at amino acid positions 112 and 158.41 Here, APOE is linked to early-onset autosomal dominant Alzheimer disease.