NHLRC1 and Lafora disease: To study the effectiveness of malin restoration in a scenario more representative of a patient with clinical symptoms of Lafora disease, we next induced malin expression in 11-month-old malinKO+OE mice, a time-point at which malinKO mice present a high abundance of LBs and a strong inflammatory response, as reflected by profound astrocytosis and microgliosis.10,11,26 Mice were analysed 4 months after the induction of malin expression (referred to as malinKO+OE[11+4]).