A different approach was used when constructing Ad5-10miR145T by inserting several binding sites for a tumour suppressor miRNA (miRNA-145; miR-145) to regulate E1A expression (Table 5) (Bader et al., 2010; Ji et al., 2017; Shayestehpour et al., 2017). Here, DHTKD1 is linked to neoplasm.