Also, the results of MTS cell viability assay and transwell matrigel invasion assay also showed that 740 Y-P increased the growth and invasion of CAL-27 cells induced by SQLE knockdown, whereas LY294002 significantly abrogated the growth and invasion of SCC-9 cells promoted by SQLE overexpression, suggesting PI3K/Akt signaling is involved in the SQLE upregulation-mediated HNSCC tumor growth (Figures 7E-F). Here, AKT1 is linked to head and neck squamous cell carcinoma.