Last but not the least, the results of in vivo tumor growth and metastasis also showed that 740 Y-P increased the tumor growth and pulmonary metastases of CAL-27 cells induced by SQLE knockdown, whereas LY294002 significantly abrogated the tumor growth and pulmonary metastases of SCC-9 cells promoted by SQLE overexpression, demonstrating that SQLE promotes HNSCC tumor growth and metastasis via activating PI3K/Akt signaling (Figure 7H-K and Figure S4C-H). This evidence concerns the gene AKT1 and head and neck squamous cell carcinoma.