Consistently, NUF2 overexpression plasmid (pcDNA3.1-NUF2), which significantly increased NUF2 mRNA and protein levels in ccRCC cells, generated opposite effects; overexpression of NUF2 could sharply augment cell proliferative, migratory, and invasive capacities (Figure 2E-2H). Here, NUF2 is linked to nonpapillary renal cell carcinoma.