NUF2 and nonpapillary renal cell carcinoma: Next, qRT-PCR and western blot assays verified that depletion of NUF2 decreased HMGA2 mRNA and protein levels in 769-P and ACHN cells, and the opposite effect was observed in NUF2-overexpressed cells, indicating that HMGA2 may be one of the target genes regulated by NUF2 in ccRCC (Figure 3C, 3D).