Using the tandem monomeric mRFP-GFP tagged LC3, the decreased GFP+RFP+ signal and increased GFP-RFP+ signal were observed in CRC cells overexpressing RAB7A under OXI treatment (Fig. 6D-G), which demonstrated that OXI inhibits the fusion of lysosome and autophagosome through downregulating RAB7A. The gene discussed is MAP1LC3A; the disease is colorectal carcinoma.