In addition, we used tandem monomeric mRFP-GFP tagged LC3 plasmid (in which the GFP fluorescence of mRFP-GFP-LC3B tandem was quenched whereas RFP fluorescence was stable in acidic compartments) to detect the formation of autolysosomes in OXI-treated CRC cells. This evidence concerns the gene MAP1LC3B and colorectal carcinoma.