Additionally, semiquantitative analysis of ACAN, Col1, and Col2 content by Western blotting also revealed that ACAN and Col2 were expressed at higher levels in NPC spheroids than in NPCs (Figure 8E-F), whereas Col1 was expressed at higher levels in NPCs than in NPC spheroids (Figure 8E-F). The gene discussed is ACAN; the disease is nasopharyngeal carcinoma.