Epilepsy is a complex neurological disorder so to identify the exact genetic cause we used this technique and identified two novel missense mutations in the SCN1A gene along with frameshift mutation in CACNB4. This study provides all possible genetic variant in epilepsy and related to disease diagnosis and prognosis and further, enable us to provide a foundation for understanding the critical genomic regions that will help to solve the epileptic problem in Saudi patients. The gene discussed is CACNB4; the disease is epilepsy.