ANXA5 and breast carcinoma: To understand the impact of longer treatment of abemaciclib and palbociclib on their efficacy in breast cancer cell lines, a time course (2, 6 and 9 days) at 50, 100 and 250 nM was performed to evaluate cells undergoing early (PI-/Annexin V+) and late (PI+/Annexin V+) apoptosis, and cell death (PI+/Annexin V-) by flow cytometry (Figure 4).