To explore the potential mechanism of P4HA1 in RCC, we transfected the 786-O and ACHN cells with OE-P4HA1 and assessed the mRNA and protein levels of epithelial–mesenchymal transition (EMT)–related genes by qRT-PCR, Western blot, and immunofluorescence. This evidence concerns the gene P4HA1 and renal cell carcinoma.