After exclusion criteria were applied, six genes (GBA, ATG10, TRIM27, CD160, ISYNA1, RAD51B) were selected for validating the associations between DNA methylation and BC with MassARRAY EpiTyper assays in validation I (48 sporadic BC cases and 48 matched cancer-free female controls) (Supplementary Table S1). This evidence concerns the gene ISYNA1 and cancer.