The results showed that overexpression of miR-19b-3p in vivo can protect mice from CVB3-induced VM, and manifested as increased body weight, prolonged survival time, increased LVEF and LVEF, decreased inflammation, decreased levels of CKMB, LDH, and AST, and the production of IFN-γ, IL-6, and IL-10 reduce; it suggested that CVB3-induced myocarditis was significantly relieved, showing that miR-19b-3p could significantly alleviate CVB3-induced myocarditis. The gene discussed is IL10; the disease is myocarditis.