To validate the different expression levels of the five genes (SOCS2, EGR1, RRM2, TPP1, and C11orf54) based on our TILTregSig in Tregs between healthy donors and prostate cancer patients, we obtained Tregs excavated from PBMCs of 3 healthy donors and 3 prostate cancer patients, and carried out Western blot analysis. This evidence concerns the gene EGR1 and Familial prostate cancer.