GUSB and hepatocellular carcinoma: qRT-PCR was used to examine these miRNAs expression in HCC cells cultured with sh-NC or sh-GUSB and the results indicated that miR-513a-5p expression was both downregulated in Hep-3b and HCC-LM3 cells, whereas miR-570-5p, miR-200a-3p, miR-34a-5p, and miR-146a-5p were not expressed as expected (Figures 4A–E).