On day 20, the tumor volume and weight of the sh-GUSB group were significantly smaller than those of the sh-NC group, and the tumor volume and weight of the sh-GUSB +anti-PD1 group were significantly smaller than those of the sh-GUSB group and sh-NC +anti-PD1 group (Figures 5C, D). Here, PDCD1 is linked to neoplasm.