To investigate the effect of GUSB on the immune microenvironment of HCC in vitro, human HCC cells (Hep-3B, HCC-LM3) were co-cultured with sh-GUSB or sh-NC, and antigen-specific CD8+ T cells isolated from 3 PBMC specimens were co-cultured. Here, CD8A is linked to hepatocellular carcinoma.