In our study, we found that knocking down of GUSB promoted the CD8+ T-cell exhaustion marker expression co-cultured with antigenspecific CD8+ T cells separately from PBMC specimens, and down-expression of GUSB prevented proliferation, invasion, and migration of human HCC cells and upregulated PD-L1 expression by decreasing miR-513a-5p expression. The gene discussed is CD8A; the disease is hepatocellular carcinoma.