Our results provide additional supportive evidence that the humanized eNAMPT-neutralizing biologic, ALT-100 mAb is highly effective in attenuating the magnitude of eNAMPT/TLR4 inflammatory cascade activation in the combined ARDS and VILI porcine model (Quijada et al., 2021; Bermudez et al., 2022). Here, TLR4 is linked to acute respiratory distress syndrome.