Our TWAS study identified 6 genes significantly associated with CRC (GREM1-AS1, C11orf53, POU5F1B, DUSP10, COLCA1, and COLCA2) with a Bonferroni-corrected threshold of P < 1.11 × 10−6 (0.05/44,953). Here, POU2AF2 is linked to colorectal carcinoma.