The expression of HMGB1 in the KYSE180, ECA109, TE1, KYSE150, KYSE510, and KYSE30 ESCC lines was determined in vitro by Western blotting, and the results revealed lower expression levels of HMGB1 in the KYSE30, and KYSE510 cell lines compared with the other cell lines (Figure 2A). This evidence concerns the gene HMGB1 and esophageal squamous cell carcinoma.