<h4>Background</h4>This study aims to assess the efficacy and safety of different doses of intravenous tissue-type plasminogen activator (tPA) for acute ischemic stroke (AIS) by adopting a network meta-analysis (NMA).<h4>Methods</h4>Studies comparing different doses of tPA in AIS were identified by retrieving electronic databases. The gene discussed is PLAT; the disease is androgen insensitivity syndrome.