Since we wanted to focus on the role of Plts as NET inducers in an HUS context, and evidence possible potentiating effects caused by Plts, in the first part of our study, we decide to use lower, non-netotic doses of LPS and Stx2 and also washed treated Plts before co-incubation with PMN to avoid the direct impact of free toxins on PMN. The gene discussed is STX2; the disease is hemolytic-uremic syndrome.