Due to the need for very sensitive methodologies for the early detection of cancer in liquid biopsy [5], we used ultrasensitive droplet digital PCR (ddPCR) for the methylation analysis of LINC00473. Consistent with our previous results in tissue and cfDNA, the methylation levels of LINC00473 were significantly higher in ACP and CRC than in confirmed healthy controls (Fig. 4E). The gene discussed is PDE10A; the disease is colorectal carcinoma.