Staining with the VSMCs differentiation marker TAGLN was further performed, revealing that αSMA and TAGLN double‐positive VSMCs located at calcium deposits area also expressed Runx2 (red arrow in Figure 2C–H, K), which suggested that VSMCs were involved in the calcification process of the venous wall after thrombosis. Here, RUNX2 is linked to deep vein thrombosis.