Apart from the significant main effects of sex (F (1, 63) = 154.1, p < 0.0001) and protein (F (1, 63) = 5.061, p = 0.028), a significant effect of pH (F (1, 63) = 5.402, p = 0.0234) was also observed in HF diet fed groups for serum insulin levels (Table 4). This evidence concerns the gene INS and hydrops fetalis.