Furthermore, because tau protein is hyperphosphorylated and abnormally accumulates in axons, dendrites, and cell bodies in subjects with AD, Western blot analysis was performed to detect the levels of tau protein; the level of tau phosphorylation was substantially increased in the APPsw-positive cells compared to GFP-positive cells (p < 0.05) (Figure 1D). Here, MAPT is linked to Alzheimer disease.