Thus, we used Masson staining and Sirius Red staining to detect the collagen deposition in the liver, finding clearly visible signs of that in the obese TRIM67 WT liver but not in the obese TRIM67 KO liver (Figure 4A,B), which indicated an attenuated hepatic fibrosis induced by obesity after TRIM67 deficiency. The gene discussed is TRIM67; the disease is obesity due to melanocortin 4 receptor deficiency.