In mouse melanoma models with BRAFV600E mutation and PTEN loss, Deken and colleagues showed that PI3Ki + BRAFi + MEKi (triplet therapy) resulted in slightly lower T cell infiltration into tumors when compared with BRAFi + MEKi (doublet therapy) alone, though both groups had significantly increased infiltration compared to untreated mice [58]. This evidence concerns the gene PTEN and melanoma.