To further characterise the role rs929039, rs9622682, and rs4820293 in Gal1 protein expression, we evaluated its levels by western blot (WB) in lymphocytes isolated from peripheral blood of 86 EA patients selected according to the presence of homozygous genotypes from the SNPs rs929039 (TT/CC), rs9622682 (GG/AA) and rs4820293 (GG/AA). This evidence concerns the gene LGALS1 and Esophageal atresia.