In the context of the development of a 3D cortical spheroid model for cerebral ischaemia, Ko et al. confirmed that 3D cell culture models represent better normal brain models, since the neural cells in 3D maintained their healthy physiological morphology of a less activated state and suppressed mRNA expressions of pathological stroke markers S100B, IL1-β and MBP [151]. Here, S100B is linked to stroke disorder.