On the other hand, the neuroprotective role of NCKX2 in brain ischemia [12] is further supported by the results of the present study in which the neuroprotective effect exerted by preconditioning on tMCAO was prevented by the genetic ablation of nckx2, thus suggesting that the presence of NCXK2 renders the brain more resistant to brain ischemic damage. The gene discussed is SLC24A2; the disease is brain ischemia.