In a validation experiment performed on primary astrocytes of mice and a larger group of patients (NMO: n= 18, HCs: n = 15) using rt-QPCR analysis, we confirmed that the expression level of BID was significantly increased in astrocytes cultured with sera of seropositive NMO patients compared to HCs (1.02 ± 0.6 RQ and 0.54 ± 0.22 RQ, p = 0.0037, Figure 2b). This evidence concerns the gene BID and neuromyelitis optica.