MAP1LC3A and hereditary elliptocytosis: For the higher dose of HE, the clear overexpression of p62 throughout culture, together with no clear increase in LC3-IIB conversion despite increase in LC3 towards end of differentiation, ERK 1⁄2 phosphorylation reduction (at day 5) and a decrease in number of apoptotic cells suggest that HE may present the ability to prolong the survival of those cells; however, they are not able to sustain autophagy which can impair full osteoclast differentiation and function [40].