KCNN4 and neoplasm: Then, in the gradient dilution model of NOD-SCID mouse, to further explore the role of KCNN4 in tumor initiation, CSCs enriched from tumorspheres of lv-ctrl, ov-KCNN4, and sh-KCNN4-2 were inoculated in each mouse individually at different numbers: 2 × 102, 2 × 103, 2 × 104, and 2 × 105.