ACTB and melanoma: Considering a p-value ≤ 0.05 to assess the significance, four of the six selected DEGs (ACTB, RPS17, RPL31, and RN28S1N5) showed statistically significant differences between the melanoma samples and healthy melanocytes, with fold change directions (expressed as relative expression fold changes performed by the ΔΔCT method) consistent with the microarray screening results (Supplementary Figure S2).